This is a description of the major changes in Volocity 6.2 and contains some guidance for users upgrading from a recent, previous version. For a full list of all the changes in Volocity 6.2 please see the release notes. For information on how to use Volocity see the Volocity User Guide or Help within the application.
Support for ‘relative position of field’ information from Harmony/Operetta
Volocity now reads the position of fields within wells from images exported from Harmony. This is of particular benefit when stitching overlapping tiles to make high resolution images larger than the field of view since stitching in Volocity is accelerated when the image bounds are known.
Support for Windows 8 and Mac OS 10.8
Volocity 6.2 is validated for use with Windows 8 and Mac OS 10.8. Please note that although the software has been validated, you are advised to check the availability of drivers for your hardware and compatibility of system components.
The Imaging License Server (ILS) on Windows 8 or Mac OS 10.8 requires ILS version 2.2.
Basic measurement functionality is now available in Volocity Visualization. Simple line, point and region of interest measurements are enabled in Volocity Visualization whereas previously they were only available with Volocity Quantitation. Point counts, line lengths, region volume and intensity measurements are displayed.
Now, even if you have a Volocity Visualization license on its own a measurement tab will be available.
Selecting the Measurements tab will show measurements, and summaries of measurements, made by drawing regions of interest, lines or points using the tools available in this view. Measurements may be exported by selecting the tab delimited or comma separated text options in the “Export…” dialog.
Volocity Quantitation is still required for automatic object detection, finding cells and intracellular structures, relational measurements, distance measurements, correlative analysis of colocalization, ratiometric analysis and FRET analysis.
Restoration is an ideal complement to many imaging systems, further increasing the resolution of images, including confocal images. In Volocity measured or calculated point spread functions (PSFs) may be used and in Volocity 6.2 PSFs specifically for deconvolution of images from PerkinElmer High Content Instrumentation may be calculated. PSFs are created from the Actions menu. Select “Create New”, and “Calculated PSF…” You can then choose the PSF type according to the system used.
Naming of dual cameras
On systems using dual cameras, both cameras can now be given user defined names to clarify workflows. Names representing camera position or wavelength sent to the camera are reflected throughout the interface. Set camera names in the “Preferences...” dialog under the “Devices” setting.
New hardware support
The Hamamatsu sCMOS camera the ORCA-Flash4.0 camera is now supported in Volocity Acquisition and on UltraVIEW VoX systems. The high speed and high resolution of this camera means 64-bit Windows is recommended for best results.