The Scripps Research Institute

The Scripps Research Institute

From left: Jeanette Ampudia, Jean-Pierre Clamme, Vasily Rybakin, Nick Gascoigne, Guo Fu, John Hoerter

The Scripps Research Institute is one of the largest, private, non-profit research organizations in the USA. The institute has always stood at the forefront of basic biomedical science, a vital segment of medical research that seeks to comprehend the most fundamental processes of life. In just five decades the Institute has established a lengthy track record of major contributions to the betterment of health and the human condition.

The Institute has become internationally recognized for its basic research into immunology, molecular and cellular biology, chemistry, neurosciences, autoimmune diseases, cardiovascular diseases, virology and synthetic vaccine development.

Professor Nick Gascoigne founded his laboratory in the department of immunology at the Scripps Research Institute in California in 1987. Nick gained his PhD at University College London studying cellular immunology and furthered his research in molecular immunology working on T-cell receptor genes at Stanford.

The Gascoigne Lab focuses on T cell activation using imaging amongst other techniques. Research into T cell activation is highly important in the understanding of effective immune response systems. The group is currently working on T cell recognition of high and low affinity antigens, to understand how T cell behavior is influenced by the affinity of the interaction between the T cell receptor and the MHC-peptide ligand.

The team is using Volocity primarily to analyze data from their two-photon microscope. Two-photon microscopy allows visualization of cells deep in tissue, so that T cells interacting with antigen presenting cells during an immune response, or thymocytes interacting with thymic stromal cells during development, can be viewed.

Nick says "We found the 3D Tracking feature to be a very efficient and easy to use tool for the monitoring of T cell movement in the lymph node in response to antigenic stimulation"

If you would like to read more information about the Gascoigne Laboratory please visit their website

The first image shows a 2D projection of T cells in a mouse lymph node. The red cells are antigen-specific T cells (SNARF labeled). The green cells are GFP-expressing dendritic cells loaded with antigenic peptide. Tracks are shown for the movement of some of the T cells. (Imaged by Dr. Jean-Pierre Clamme)
 
The second shows a 3D rendering of the same time point, so that the T cells move in three dimensions within the lymph node.