Research group of Dr Jackie Vogel, McGill University

Research group of Dr Jackie Vogel, McGill University

Dr Vogel is Assistant Professor in the Department of Biology at McGill University and is a CIHR (Canadian Institutes of Health Research) New Investigator. As a member of the Developmental Biology Research Initiative (DBRI), Dr Vogel is developing facilities for low light 6D imaging of living cells and in vivo post-translational modification analysis. Currently, she is the coordinator of the DBRI Cell Imaging and Analysis Network (CIAN). When complete, the CIAN will be the largest deployment of networked imaging and analysis systems of its kind in Canada.

Jackie Vogel received a PhD in Cell Biology from the University of Kansas, where she studied meiotic centrosome maturation in the lab of Robert Palazzo. She examined the regulation of conserved proteins required for microtubule assembly and organization in budding yeast as a NIH-NSRA and Warner Post-Doctoral Fellow in Michael Snyder’s lab at Yale University. She joined the Department of Biology at McGill in August 2002.

Cell division requires temporal and spatial coordination of nuclear and cytoplasmic events. This coordination is particularly important in polarized cells, in which proper positioning of the nucleus must occur prior to partitioning of genetic material and cytokinesis.

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Nuclear positioning and chromosome segregation requires dynamic re-organization of microtubules and F-actin during the cell cycle. The budding yeast Saccharomyces cerevisiae is highly suited for genetic, biochemical and cytological studies and undergoes polarized growth in the form of a bud or a mating projection.

Research in the Vogel lab focuses on several aspects of regulation of microtubule assembly, organization and function during the budding yeast cell cycle: 1) regulation of microtubule organization during spindle positioning, 2) chromosome segregation and kinetochore function, 3) mechanisms of gamma-tubulin-dependant microtubule assembly, and 4) global analysis of the role of protein phosphatases in vivo.

Jackie’s lab uses a Spinning Disk confocal configuration that consists of a Leica DM 6000 and Volocity 3DM and a deconvolution system on a Nikon TE 2000, which is powered by Volocity 3DM and Openlab. The laboratory uses two Volocity and Openlab workstations using the Improvision License Server plus CIAN workstations. Currently the ILS is configured with 21 Volocity licenses and 6 Openlab licenses and is the largest ILS system to date.

Jackie writes: “We have two microscope systems that use Openlab and Volocity 3DM to study cytoskeletal dynamics and checkpoint function in budding yeast. We use Volocity 3DM for acquisition, and perform analysis using Volocity Classification (for example tracking +tip proteins on microtubules or GFP labeled centromeres), Volocity Visualization for rendering 4D (single channel) and 6D (dual channel) time-lapse experiments into movies. Volocity Restoration is used for deconvolution of 3D volumes. Recently, Volocity and Openlab have been used in the lab for the functional characterization of an essential kinetochore protein (published [Cell Cycle 4:10, e128-e135]), and to examine the role of gamma-tubulin in the function of microtubule +tip proteins required for spindle positioning (submitted manuscript).

"We find using Volocity and Openlab for our imaging experiments is ideal for our needs and the server solution provides the flexibility that CIAN demands." Dr. Vogel was recently featured on an Apple webcast for Cost Effective Storage Deployments for Research Computing, titled "Xsan and Xserve as a platform for a cell imaging and analysis network" (http://seminars.apple.com/seminarsonline/researchstorage/apple/)

Loss of Bub3-VFP localization (green) at kinetochores (adjacent to spindle poles; yellow) in the presence of separated sister chromatids (red) indicates failure to maintain metaphase arrest in ame1 mutant cells.