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Latest Tips

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Movie making in Volocity: a video tutorial

Movie making in Volocity: a video tutorial

Hi folks,

Well, I have something a bit different for you this month. As my tips that include aspects of movie making always go down well, I have decided to go back to basics and show you how to make your own movie in Volocity from...Read More

How do I make a movie that shows tracking of objects over time?

How do I make a movie that shows tracking of objects over time?

Everyone loves a good movie and a question that I am frequently asked is how I make movies in Volocity that show tracking of objects as they move in time. These movies help to give a more detailed insight into biological processes and they are great for incorporating...Read More

Create a movie which plays through your Z planes at a chosen XY angle.

Create a movie which plays through your Z planes at a chosen XY angle.

A couple of weeks ago, one of my colleagues asked me how he could create a movie in Volocity which would allow him to play through his Z-stack in the 3D planes view using a chosen XY angle, as he wanted...Read More

Minimizing axial elongation of structures

Minimizing axial elongation of structures

I have a short tip for you this month, inspired by a conversation with one of my grad students.

In our lab, we use fluorescent beads to test the performance and calibration of our system and to measuring point spread functions (PSFs) to model the behaviour of light. The...Read More

Technical Notes

Title Product Modification Date Views
Columbus Software Installation Guide Columbus 02/10/2014 3250
Columbus 2.5 and Acapella Script Compatibility Columbus 19/09/2014 2086
Volocity Supported Hardware Volocity 19/07/2013 21288
What's New in Volocity 6.3 Volocity 17/07/2013 2764
Exporting TIFF Data from Volocity Volocity 01/07/2013 2615
Olympus IX83 Driver Installation Volocity 08/05/2013 3161
Workflows for In-depth High Content Analysis Columbus,Volocity 23/01/2013 2927
Data Transfer from Operetta to Volocity Volocity 21/01/2013 2772
Data Transfer from Columbus to Volocity Columbus,Volocity 21/01/2013 2680
Data Transfer from Operetta to Columbus Columbus 21/01/2013 2904
Data Transfer from Opera to Columbus Columbus 21/01/2013 2736
Data Transfer from Opera to Volocity Volocity 21/01/2013 2630
System Requirements for Volocity Product Range Volocity 22/11/2012 19876
Using the Nikon TE2000 PFS with Volocity Acquisition Volocity 22/11/2012 5310
Using the Leica DMI6000 AFC with Volocity Acquisition Volocity 22/11/2012 3782
Using the Nikon TiE PFS with Volocity Acquisition Volocity 21/11/2012 5098
What's New in Volocity 6.2? Volocity 21/11/2012 2933
Keyboard Shortcuts in Volocity Volocity 11/06/2012 4030
What's New in Volocity 6.1? Volocity 23/04/2012 3131
Skeletal Length Measurement in Volocity 6.0 Volocity 13/09/2011 3448
What's New in Volocity 6.0? Volocity 13/09/2011 3473
Openlab Supported Hardware Openlab 15/03/2011 16804
Configuring Volocity Acquisition Systems for Stitching Volocity 15/03/2011 4499
Creating and Editing Complex ROIs in Volocity 5.5 Volocity 10/01/2011 3861
Using the Zeiss AxioObserver Definite Focus with Volocity Acquisition Volocity 13/12/2010 5505
What's New in Volocity 5.5? Volocity 09/12/2010 4050
Using Lightpaths with the UltraVIEW Volocity 18/11/2010 3725
Calibrating PK Unit on Upgraded UltraVIEW LCI System Volocity 11/08/2010 3935
Volocity Demo Getting Started Guide Volocity 04/08/2010 7819
Volocity Getting started Volocity 04/08/2010 4362
Volocity and Windows 7 Volocity 28/05/2010 4750
Using the Olympus IX81 ZDC with Volocity Acquisition Volocity 27/05/2010 5719
Limitations of Hamamatsu ImagEM "Real-time Image Processing" Features Other 07/12/2009 4874
UltraVIEW VoX - Optimizing Acquisition Frame Rate Volocity 09/07/2009 4832
Volocity for Windows x64 Volocity 15/04/2009 6801
Volocity Surface Area Measurements Volocity 14/01/2009 4713
Volocity and Windows Vista Volocity 17/11/2008 5970
ILS System Requirements ILS 13/08/2008 7086
Additional TIFF Information in Phylum and Volocity Phylum and Volocity 11/06/2008 6920
System Specifications for Imaging Computing Server Volocity 02/04/2008 6188
Openlab 5.5 Openlab 27/03/2008 5163
Volocity for Windows: Optimizing performance Volocity 28/02/2008 7954
Spectral Separation of Monochrome Images Using Volocity 4 Volocity 13/08/2007 5270
Volocity and Neurolucida Volocity 30/03/2007 5461
Openlab 5: Color Tables and Automator Items Openlab 21/12/2006 5802
Finding Dirt and Debris in your Microscope Other 24/11/2006 7423
Diagnosing Microscope Drift Problems Openlab 22/11/2006 9881
Improvision Micro-Shutter performance Phylum Live 02/05/2006 4472
Openlab 5: New Location for Color Tables, Automator Menu Items and Automator Subroutines Openlab 28/04/2006 5667
Openlab and Mac OS X Openlab 16/01/2006 13671
Improvision Micro-Shutter performance Other 04/01/2006 9433
A study of fluorescence standards confirms that OptiGrid confocal images are suitable for quantitative microscopy Phylum Live 22/08/2005 4681
How do I transfer files between a PC and a Mac? Openlab 19/08/2005 14277
How does Openlab work with different image formats? Openlab 19/08/2005 6184
Which image files are compatible with Openlab? Openlab 19/08/2005 7508
How can I open TIFF Images with Openlab? Openlab 19/08/2005 6206
Why does there appear to be degradation to the resolution of the graphics in an annotation layer compared to that of a measurement overlay? Openlab 19/08/2005 6347
How can I create my own columns in the Advanced Measurements table? Openlab 19/08/2005 5925
What is a typical practical value for the accuracy and repeatability of the PIFOC piezo focus drive? Openlab 19/08/2005 6307
How do I export images from Openlab as individual TIFFs for use in other graphics applications? Openlab 19/08/2005 7486
How do I optimise memory in Mac OS 9 for use with Openlab? Openlab 19/08/2005 6081
How do I save the color LUT when exporting images into Adobe Photoshop? Openlab 19/08/2005 6995
Why is the device connected to the Serial Port of my computer not communicating with Openlab? Openlab 19/08/2005 6302
Why am I only able to capture images at a reduced size rather than full image size? Openlab 19/08/2005 5453
Controlling the VMM-D3 Three Channel Shutter Driver using Openlab Automator Serial Communications Openlab 19/08/2005 6731
Volocity for Windows - Dongle Installation Volocity 19/08/2005 7082
Using Openlab Layer User Data to set properties within Volocity Volocity 19/08/2005 5731
How do I run serial devices from a Mac which has only USB ports? Openlab 26/07/2005 5951
Year 2000 Statement Openlab 26/07/2005 5896
Achieving accurate color reproduction for brightfield microscopy using a color camer. Openlab 25/07/2005 6108
How do I make a scale bar to stamp on my images in Openlab? Openlab 25/07/2005 9666
Openlab Raw Format Openlab 09/11/2004 13597
Openlab Module - User Data Openlab 11/03/2004 5600
Ludl Controller Configuration Openlab 11/03/2004 7248
How do I density slice an image? Openlab 12/02/2004 8463
How do I edit the settings on my Openlab Filterwheel palette? Openlab 12/02/2004 5806
How do I set up the Dage DC330 color camera for transmitted light microscopy in Openlab? Openlab 12/02/2004 6050
Openlab User Interface Concepts Openlab 12/02/2004 5573
Can Phylum archive movie files? Phylum 12/02/2004 6127
How do I calibrate my TILL Photonics Polychrome monochromator in Openlab? Openlab 12/02/2004 7987
How do I use the CRI Micro Color filter with Openlab? Openlab 12/02/2004 6515
How do I prevent erratic timings when capturing large sequences of images in Openlab? Openlab 12/02/2004 5646
Does the Openlab Automator support array variables? Openlab 12/02/2004 5844
How do I superimpose results from the Measurements table onto an image layer? Openlab 12/02/2004 6100
How can I open images acquired with older Improvision imaging products in Openlab? Openlab 12/02/2004 5923
Power Macintosh G3 and G4 computers using Mac OS 9 with the National Instruments PCI 1200 card. Openlab 12/02/2004 6029
How do I measure the optical density of objects using Openlab? Openlab 12/02/2004 8775
How do I set up and use the the multi-user facility in Openlab? Openlab 12/02/2004 5248
How can I record a variable in an Openlab automation to be exported to a data analysis program such as Excel? Openlab 12/02/2004 5584
Where can I find the Openlab serial number? Openlab 12/02/2004 6173
Why do Openlab images look poor on the monitor? Openlab 12/02/2004 5349
Why does my automation refuse to stop during a Tell or Input task? Openlab 12/02/2004 5440
Why does my automation refuse to pause during a Tell task? Openlab 12/02/2004 5445
How can I determine the exact degree of image registration that is needed to re-align my multilabelled fluorescent image Openlab 12/02/2004 6728
Why can't I import Adobe Photoshop files into Phylum? Phylum 12/02/2004 5774
How do I move images from different Openlab documents into one Openlab document? Openlab 12/02/2004 5509
Keyboard Shortcuts for use with Openlab. Openlab 12/02/2004 6176
How do I convert the Openlab layer time stamp from days:hours:mins:sec into seconds so that I can use the data in Excel? Openlab 12/02/2004 5786
Why can't I annotate my images in white text using a transparent annotation layer in Openlab? Openlab 12/02/2004 5547
How do I track the speed and position of cells in a time lapse sequence with Openlab? Openlab 12/02/2004 6017
How do I make a QuickTime movie in Openlab? Openlab 12/02/2004 5829
How can I make an automation go faster? Openlab 12/02/2004 5449
Why can't I annotate my images using the Paint Tools palette? Openlab 12/02/2004 5745
Why does the image sequence that I saved as a QuickTime movie look pixelated? Openlab 12/02/2004 5919
How can I export graphs generated in Openlab into other applications ? Openlab 12/02/2004 5885
How do I reset the Point Counter so that I can begin a new count on each layer? Openlab 12/02/2004 5636
I am using OpenLab with Mac OS 9.2.2 and I am repeatedly getting out of memory errors when I try different commands. What should I do? Openlab 12/02/2004 5570
I am using binning to increase the sensitivity of my camera. I can view the image in preview mode but get an error message when I try to capture the image. Openlab 12/02/2004 5535
I’m selecting an ROI measurement from the Measurements Table, but Openlab is not displaying either the ROI or the image that it corresponds to in the document window. Openlab 12/02/2004 5657
I have downloaded the latest version of Openlab from www.improvision.com. Now what do I do? Openlab 12/02/2004 5712
How do I change the color of the paint tools? Openlab 12/02/2004 5530
What should I know about the 3D Restoration module? Openlab 12/02/2004 5688
How can I focus the SPOT RT camera quickly? Openlab 12/02/2004 6143
How can I play a movie in Phylum which doesn't include all of the images in the folder? Phylum 12/02/2004 5497
Why doesn't the software recognize my camera after I have installed a new version of Openlab? Openlab 12/02/2004 5845
Why can’t I apply a color look up table (LUT) to my merged images? Openlab 12/02/2004 5863
Which way should filters go into my filter wheel? Other 12/02/2004 5612
I can't see the line that has been drawn when I create an ROI or use the measurement tools in Openlab. Can I change the color? Openlab 12/02/2004 5386
When I use the RGB merge to merge a slightly opaque layer with other layers, why is the resultant image of poor quality, and why is detail in the opaque layer lost? Openlab 12/02/2004 5837
Why is only the Layers Manager palette available when I start up Openlab? Openlab 12/02/2004 6010
Can I use 8 or 12 bit TIFF files generated by other systems within Openlab? Openlab 12/02/2004 5656
Why am I getting a grainy black and white image on my color CoolSNAP camera? Openlab 12/02/2004 5902
My camera has non symmetrical pixels. Which value do I enter for the pixel size in the deconvolution dialog box? Openlab 12/02/2004 5488
How do I use the AxioCam camera with Openlab? Openlab 12/02/2004 6009
Zeiss Axioplan 2ie and Axiovert 200M behave strangely with Openlab 2 Openlab 12/02/2004 7476
Openlab 3 - The Filters and Shutters module now supports 'link to video' for shutters. Openlab 12/02/2004 5601
Increased functionality for the Automator Lite in Openlab 3 Openlab 12/02/2004 5406
What features of the Hamamatsu 4742-95ER (Orca ER) are now supported in Openlab 2.2.5r2? Openlab 12/02/2004 6731
Auto Exposure in Openlab Openlab 12/02/2004 6142
Preferences Issues When Moving Between Openlab versions 2 and 3. Openlab 12/02/2004 5500
Openlab 3 - Auto Focus now supported for motorised focus drives Openlab 12/02/2004 5946
Using Roper (Princeton Instruments) cameras with the PVCAM Video module Openlab 12/02/2004 8767
Can I make a binary mask using the Binary Operations module? Openlab 12/02/2004 6310
Why is the integration of my Hamamatsu C5810 camera not synchronised within an automation? Openlab 12/02/2004 6392
How do I save spatial calibrations for different objectives in Openlab? Openlab 12/02/2004 5499
Which cameras will speed up significantly when using the Automator "Capture Multiple" task? Openlab 12/02/2004 5698
What is the UV throughput of the fiber optic supplied with the TILL monochromators and are they solid quartz? Openlab 12/02/2004 5960
How do I use Openlab to measure the greyscale intensity values for a number of different ROIs over the course of a timelapse experiment (i.e. over several layers)? Openlab 12/02/2004 5818
I lost the power to the Openlab acquisition system and lost the image file I had captured. Can I get it back? Openlab 12/02/2004 5637
Can I use more than one Ludl controller with Openlab? Openlab 12/02/2004 5760
How do I remove the number from my cell tracking overlay in Openlab? Openlab 12/02/2004 5531
I cannot get communication with my ORBIT 1 controller in Openlab. Openlab 12/02/2004 6023
How do you make a 3D profile in Openlab? Openlab 12/02/2004 5540
How can I measure grayscale intensity values over an image using the same ROI? Openlab 12/02/2004 7371
When I draw an ROI on an image, it won't retain the shape I have drawn. Openlab 12/02/2004 5538
How do I change size of image in order to speed up capture and/or processing time? Openlab 12/02/2004 5728
Changes in Openlab 3 to the Automator Controls palette Openlab 12/02/2004 5558
"Save as Multiple" and file naming in Openlab Openlab 12/02/2004 6079
How do I measure the total intensity of a cell or ROI in Openlab? Openlab 12/02/2004 7535
What version of Mac OS can I use with Openlab? Openlab 12/02/2004 5833
Openlab 3.0.2 - Target Image Window Automator task - new option Openlab 12/02/2004 5828
Orbit Focus Drives - Preferences Openlab 12/02/2004 5892
Volocity 3D Rendering - how to export a 2D view Volocity 12/02/2004 6075
Write to User Notes Automator task - Tab function Openlab 12/02/2004 6181
Open Image Document Automator Task Openlab 12/02/2004 5741
Save Document Automator Task - format options Openlab 12/02/2004 5823
When using the "Volume Deconvolution" module in Openlab, the final deconvolved image does not resemble the image in the preview window. Openlab 12/02/2004 5468
Using Volocity, I have enhanced my images by using the Auto Contrast function but my volume does not look as expected. Volocity 12/02/2004 5587
Openlab Microscope Control - Light Path Automator task Openlab 12/02/2004 5876
Set Video Control Automator task - changing check boxes and pop up choices Openlab 12/02/2004 5553
Ludl Biopoint controllers do not fully support State Saver operations. Openlab 12/02/2004 5810
Alternate Readout Mode for Photometrics PVCAM cameras, specifically the CoolSNAP HQ. Openlab 12/02/2004 6598
Why is the progress bar on the Video Controls palette always moving? It never becomes a solid block, is this correct? Openlab 12/02/2004 5375
Zeiss AxioCam color camera - White Balance for brightfield microscopy. Openlab 12/02/2004 7908
Roper Scientific Photometrics color CoolSNAP camera - White Balance for brightfield microscopy. Openlab 12/02/2004 6581
How do I connect serial devices to Macs which do not have serial ports? Openlab 12/02/2004 13810
Openlab Module - 3D Rendering - using the 'Scale with Z' option Openlab 12/02/2004 5710
Volocity - Alignment tool Volocity 12/02/2004 6543
Leica DMSTC Motorised XY Stage and CTRMIC microscopes in Openlab Openlab 12/02/2004 8545
ORBIT 1 XY Stage Openlab 12/02/2004 6554
An example automation illustrating the use of multithreading Openlab 12/02/2004 5629
An example automation illustrating the use of parallel processing and the "Key wait" task Openlab 12/02/2004 5671
How to create an automation to measure objects and put the results into a graph. Openlab 12/02/2004 5460
Openlab Module - Colocalization Openlab 12/02/2004 5970
Openlab Module - Image Splitter Openlab 12/02/2004 5656
Spatial calibration in Openlab 3.1 and higher Openlab 12/02/2004 5729
How to install Openlab Darkroom Openlab 12/02/2004 6174
QImaging Camera Firmware Upgrade Openlab 12/02/2004 6417
Openlab Ratio Module: Intensity Modulated image Openlab 12/02/2004 5812
Openlab Module - FRET Openlab 12/02/2004 5836
Elongation of Spherical Structures in Z Other 12/02/2004 6621
Volocity LE 2.5 and higher Volocity 12/02/2004 5772
Openlab Automator Serial Communications Openlab 12/02/2004 5892