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Latest Tips

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Movie making in Volocity: a video tutorial

Movie making in Volocity: a video tutorial

Hi folks,

Well, I have something a bit different for you this month. As my tips that include aspects of movie making always go down well, I have decided to go back to basics and show you how to make your own movie in Volocity from...Read More

How do I make a movie that shows tracking of objects over time?

How do I make a movie that shows tracking of objects over time?

Everyone loves a good movie and a question that I am frequently asked is how I make movies in Volocity that show tracking of objects as they move in time. These movies help to give a more detailed insight into biological processes and they are great for incorporating...Read More

Create a movie which plays through your Z planes at a chosen XY angle.

Create a movie which plays through your Z planes at a chosen XY angle.

A couple of weeks ago, one of my colleagues asked me how he could create a movie in Volocity which would allow him to play through his Z-stack in the 3D planes view using a chosen XY angle, as he wanted...Read More

Minimizing axial elongation of structures

Minimizing axial elongation of structures

I have a short tip for you this month, inspired by a conversation with one of my grad students.

In our lab, we use fluorescent beads to test the performance and calibration of our system and to measuring point spread functions (PSFs) to model the behaviour of light. The...Read More

Technical Notes

Title Product Modification Date Views
Columbus Software Installation Guide Columbus 02/10/2014 3387
Columbus 2.5 and Acapella Script Compatibility Columbus 19/09/2014 2219
Volocity Supported Hardware Volocity 19/07/2013 21427
What's New in Volocity 6.3 Volocity 17/07/2013 2870
Exporting TIFF Data from Volocity Volocity 01/07/2013 2737
Olympus IX83 Driver Installation Volocity 08/05/2013 3285
Workflows for In-depth High Content Analysis Columbus,Volocity 23/01/2013 3048
Data Transfer from Operetta to Volocity Volocity 21/01/2013 2888
Data Transfer from Columbus to Volocity Columbus,Volocity 21/01/2013 2785
Data Transfer from Operetta to Columbus Columbus 21/01/2013 3015
Data Transfer from Opera to Columbus Columbus 21/01/2013 2848
Data Transfer from Opera to Volocity Volocity 21/01/2013 2729
System Requirements for Volocity Product Range Volocity 22/11/2012 20031
Using the Nikon TE2000 PFS with Volocity Acquisition Volocity 22/11/2012 5429
Using the Leica DMI6000 AFC with Volocity Acquisition Volocity 22/11/2012 3901
Using the Nikon TiE PFS with Volocity Acquisition Volocity 21/11/2012 5212
What's New in Volocity 6.2? Volocity 21/11/2012 3039
Keyboard Shortcuts in Volocity Volocity 11/06/2012 4156
What's New in Volocity 6.1? Volocity 23/04/2012 3235
Skeletal Length Measurement in Volocity 6.0 Volocity 13/09/2011 3564
What's New in Volocity 6.0? Volocity 13/09/2011 3591
Openlab Supported Hardware Openlab 15/03/2011 16917
Configuring Volocity Acquisition Systems for Stitching Volocity 15/03/2011 4627
Creating and Editing Complex ROIs in Volocity 5.5 Volocity 10/01/2011 3965
Using the Zeiss AxioObserver Definite Focus with Volocity Acquisition Volocity 13/12/2010 5656
What's New in Volocity 5.5? Volocity 09/12/2010 4161
Using Lightpaths with the UltraVIEW Volocity 18/11/2010 3835
Calibrating PK Unit on Upgraded UltraVIEW LCI System Volocity 11/08/2010 4042
Volocity Demo Getting Started Guide Volocity 04/08/2010 7959
Volocity Getting started Volocity 04/08/2010 4468
Volocity and Windows 7 Volocity 28/05/2010 4861
Using the Olympus IX81 ZDC with Volocity Acquisition Volocity 27/05/2010 5851
Limitations of Hamamatsu ImagEM "Real-time Image Processing" Features Other 07/12/2009 4995
UltraVIEW VoX - Optimizing Acquisition Frame Rate Volocity 09/07/2009 4952
Volocity for Windows x64 Volocity 15/04/2009 6902
Volocity Surface Area Measurements Volocity 14/01/2009 4853
Volocity and Windows Vista Volocity 17/11/2008 6074
ILS System Requirements ILS 13/08/2008 7206
Additional TIFF Information in Phylum and Volocity Phylum and Volocity 11/06/2008 7034
System Specifications for Imaging Computing Server Volocity 02/04/2008 6305
Openlab 5.5 Openlab 27/03/2008 5272
Volocity for Windows: Optimizing performance Volocity 28/02/2008 8069
Spectral Separation of Monochrome Images Using Volocity 4 Volocity 13/08/2007 5378
Volocity and Neurolucida Volocity 30/03/2007 5574
Openlab 5: Color Tables and Automator Items Openlab 21/12/2006 5922
Finding Dirt and Debris in your Microscope Other 24/11/2006 7562
Diagnosing Microscope Drift Problems Openlab 22/11/2006 10126
Improvision Micro-Shutter performance Phylum Live 02/05/2006 4597
Openlab 5: New Location for Color Tables, Automator Menu Items and Automator Subroutines Openlab 28/04/2006 5781
Openlab and Mac OS X Openlab 16/01/2006 13795
Improvision Micro-Shutter performance Other 04/01/2006 9542
A study of fluorescence standards confirms that OptiGrid confocal images are suitable for quantitative microscopy Phylum Live 22/08/2005 4797
How do I transfer files between a PC and a Mac? Openlab 19/08/2005 14393
How does Openlab work with different image formats? Openlab 19/08/2005 6298
Which image files are compatible with Openlab? Openlab 19/08/2005 7629
How can I open TIFF Images with Openlab? Openlab 19/08/2005 6325
Why does there appear to be degradation to the resolution of the graphics in an annotation layer compared to that of a measurement overlay? Openlab 19/08/2005 6453
How can I create my own columns in the Advanced Measurements table? Openlab 19/08/2005 6037
What is a typical practical value for the accuracy and repeatability of the PIFOC piezo focus drive? Openlab 19/08/2005 6418
How do I export images from Openlab as individual TIFFs for use in other graphics applications? Openlab 19/08/2005 7627
How do I optimise memory in Mac OS 9 for use with Openlab? Openlab 19/08/2005 6200
How do I save the color LUT when exporting images into Adobe Photoshop? Openlab 19/08/2005 7108
Why is the device connected to the Serial Port of my computer not communicating with Openlab? Openlab 19/08/2005 6416
Why am I only able to capture images at a reduced size rather than full image size? Openlab 19/08/2005 5563
Controlling the VMM-D3 Three Channel Shutter Driver using Openlab Automator Serial Communications Openlab 19/08/2005 6844
Volocity for Windows - Dongle Installation Volocity 19/08/2005 7217
Using Openlab Layer User Data to set properties within Volocity Volocity 19/08/2005 5851
How do I run serial devices from a Mac which has only USB ports? Openlab 26/07/2005 6063
Year 2000 Statement Openlab 26/07/2005 6012
Achieving accurate color reproduction for brightfield microscopy using a color camer. Openlab 25/07/2005 6231
How do I make a scale bar to stamp on my images in Openlab? Openlab 25/07/2005 9817
Openlab Raw Format Openlab 09/11/2004 13817
Openlab Module - User Data Openlab 11/03/2004 5709
Ludl Controller Configuration Openlab 11/03/2004 7347
How do I density slice an image? Openlab 12/02/2004 8618
How do I edit the settings on my Openlab Filterwheel palette? Openlab 12/02/2004 5928
How do I set up the Dage DC330 color camera for transmitted light microscopy in Openlab? Openlab 12/02/2004 6161
Openlab User Interface Concepts Openlab 12/02/2004 5681
Can Phylum archive movie files? Phylum 12/02/2004 6232
How do I calibrate my TILL Photonics Polychrome monochromator in Openlab? Openlab 12/02/2004 8140
How do I use the CRI Micro Color filter with Openlab? Openlab 12/02/2004 6636
How do I prevent erratic timings when capturing large sequences of images in Openlab? Openlab 12/02/2004 5759
Does the Openlab Automator support array variables? Openlab 12/02/2004 5952
How do I superimpose results from the Measurements table onto an image layer? Openlab 12/02/2004 6206
How can I open images acquired with older Improvision imaging products in Openlab? Openlab 12/02/2004 6036
Power Macintosh G3 and G4 computers using Mac OS 9 with the National Instruments PCI 1200 card. Openlab 12/02/2004 6147
How do I measure the optical density of objects using Openlab? Openlab 12/02/2004 8949
How do I set up and use the the multi-user facility in Openlab? Openlab 12/02/2004 5385
How can I record a variable in an Openlab automation to be exported to a data analysis program such as Excel? Openlab 12/02/2004 5705
Where can I find the Openlab serial number? Openlab 12/02/2004 6287
Why do Openlab images look poor on the monitor? Openlab 12/02/2004 5457
Why does my automation refuse to stop during a Tell or Input task? Openlab 12/02/2004 5558
Why does my automation refuse to pause during a Tell task? Openlab 12/02/2004 5552
How can I determine the exact degree of image registration that is needed to re-align my multilabelled fluorescent image Openlab 12/02/2004 6827
Why can't I import Adobe Photoshop files into Phylum? Phylum 12/02/2004 5879
How do I move images from different Openlab documents into one Openlab document? Openlab 12/02/2004 5621
Keyboard Shortcuts for use with Openlab. Openlab 12/02/2004 6302
How do I convert the Openlab layer time stamp from days:hours:mins:sec into seconds so that I can use the data in Excel? Openlab 12/02/2004 5887
Why can't I annotate my images in white text using a transparent annotation layer in Openlab? Openlab 12/02/2004 5650
How do I track the speed and position of cells in a time lapse sequence with Openlab? Openlab 12/02/2004 6128
How do I make a QuickTime movie in Openlab? Openlab 12/02/2004 5939
How can I make an automation go faster? Openlab 12/02/2004 5562
Why can't I annotate my images using the Paint Tools palette? Openlab 12/02/2004 5857
Why does the image sequence that I saved as a QuickTime movie look pixelated? Openlab 12/02/2004 6032
How can I export graphs generated in Openlab into other applications ? Openlab 12/02/2004 6019
How do I reset the Point Counter so that I can begin a new count on each layer? Openlab 12/02/2004 5745
I am using OpenLab with Mac OS 9.2.2 and I am repeatedly getting out of memory errors when I try different commands. What should I do? Openlab 12/02/2004 5690
I am using binning to increase the sensitivity of my camera. I can view the image in preview mode but get an error message when I try to capture the image. Openlab 12/02/2004 5657
I’m selecting an ROI measurement from the Measurements Table, but Openlab is not displaying either the ROI or the image that it corresponds to in the document window. Openlab 12/02/2004 5775
I have downloaded the latest version of Openlab from www.improvision.com. Now what do I do? Openlab 12/02/2004 5828
How do I change the color of the paint tools? Openlab 12/02/2004 5642
What should I know about the 3D Restoration module? Openlab 12/02/2004 5795
How can I focus the SPOT RT camera quickly? Openlab 12/02/2004 6259
How can I play a movie in Phylum which doesn't include all of the images in the folder? Phylum 12/02/2004 5596
Why doesn't the software recognize my camera after I have installed a new version of Openlab? Openlab 12/02/2004 5956
Why can’t I apply a color look up table (LUT) to my merged images? Openlab 12/02/2004 5967
Which way should filters go into my filter wheel? Other 12/02/2004 5727
I can't see the line that has been drawn when I create an ROI or use the measurement tools in Openlab. Can I change the color? Openlab 12/02/2004 5480
When I use the RGB merge to merge a slightly opaque layer with other layers, why is the resultant image of poor quality, and why is detail in the opaque layer lost? Openlab 12/02/2004 5944
Why is only the Layers Manager palette available when I start up Openlab? Openlab 12/02/2004 6120
Can I use 8 or 12 bit TIFF files generated by other systems within Openlab? Openlab 12/02/2004 5767
Why am I getting a grainy black and white image on my color CoolSNAP camera? Openlab 12/02/2004 6012
My camera has non symmetrical pixels. Which value do I enter for the pixel size in the deconvolution dialog box? Openlab 12/02/2004 5593
How do I use the AxioCam camera with Openlab? Openlab 12/02/2004 6107
Zeiss Axioplan 2ie and Axiovert 200M behave strangely with Openlab 2 Openlab 12/02/2004 7601
Openlab 3 - The Filters and Shutters module now supports 'link to video' for shutters. Openlab 12/02/2004 5718
Increased functionality for the Automator Lite in Openlab 3 Openlab 12/02/2004 5517
What features of the Hamamatsu 4742-95ER (Orca ER) are now supported in Openlab 2.2.5r2? Openlab 12/02/2004 6865
Auto Exposure in Openlab Openlab 12/02/2004 6276
Preferences Issues When Moving Between Openlab versions 2 and 3. Openlab 12/02/2004 5609
Openlab 3 - Auto Focus now supported for motorised focus drives Openlab 12/02/2004 6058
Using Roper (Princeton Instruments) cameras with the PVCAM Video module Openlab 12/02/2004 9285
Can I make a binary mask using the Binary Operations module? Openlab 12/02/2004 6422
Why is the integration of my Hamamatsu C5810 camera not synchronised within an automation? Openlab 12/02/2004 6496
How do I save spatial calibrations for different objectives in Openlab? Openlab 12/02/2004 5608
Which cameras will speed up significantly when using the Automator "Capture Multiple" task? Openlab 12/02/2004 5809
What is the UV throughput of the fiber optic supplied with the TILL monochromators and are they solid quartz? Openlab 12/02/2004 6067
How do I use Openlab to measure the greyscale intensity values for a number of different ROIs over the course of a timelapse experiment (i.e. over several layers)? Openlab 12/02/2004 5923
I lost the power to the Openlab acquisition system and lost the image file I had captured. Can I get it back? Openlab 12/02/2004 5754
Can I use more than one Ludl controller with Openlab? Openlab 12/02/2004 5865
How do I remove the number from my cell tracking overlay in Openlab? Openlab 12/02/2004 5648
I cannot get communication with my ORBIT 1 controller in Openlab. Openlab 12/02/2004 6135
How do you make a 3D profile in Openlab? Openlab 12/02/2004 5645
How can I measure grayscale intensity values over an image using the same ROI? Openlab 12/02/2004 7509
When I draw an ROI on an image, it won't retain the shape I have drawn. Openlab 12/02/2004 5648
How do I change size of image in order to speed up capture and/or processing time? Openlab 12/02/2004 5835
Changes in Openlab 3 to the Automator Controls palette Openlab 12/02/2004 5662
"Save as Multiple" and file naming in Openlab Openlab 12/02/2004 6194
How do I measure the total intensity of a cell or ROI in Openlab? Openlab 12/02/2004 7762
What version of Mac OS can I use with Openlab? Openlab 12/02/2004 5939
Openlab 3.0.2 - Target Image Window Automator task - new option Openlab 12/02/2004 5939
Orbit Focus Drives - Preferences Openlab 12/02/2004 6016
Volocity 3D Rendering - how to export a 2D view Volocity 12/02/2004 6190
Write to User Notes Automator task - Tab function Openlab 12/02/2004 6291
Open Image Document Automator Task Openlab 12/02/2004 5843
Save Document Automator Task - format options Openlab 12/02/2004 5932
When using the "Volume Deconvolution" module in Openlab, the final deconvolved image does not resemble the image in the preview window. Openlab 12/02/2004 5580
Using Volocity, I have enhanced my images by using the Auto Contrast function but my volume does not look as expected. Volocity 12/02/2004 5697
Openlab Microscope Control - Light Path Automator task Openlab 12/02/2004 6001
Set Video Control Automator task - changing check boxes and pop up choices Openlab 12/02/2004 5648
Ludl Biopoint controllers do not fully support State Saver operations. Openlab 12/02/2004 5928
Alternate Readout Mode for Photometrics PVCAM cameras, specifically the CoolSNAP HQ. Openlab 12/02/2004 6721
Why is the progress bar on the Video Controls palette always moving? It never becomes a solid block, is this correct? Openlab 12/02/2004 5487
Zeiss AxioCam color camera - White Balance for brightfield microscopy. Openlab 12/02/2004 8070
Roper Scientific Photometrics color CoolSNAP camera - White Balance for brightfield microscopy. Openlab 12/02/2004 6709
How do I connect serial devices to Macs which do not have serial ports? Openlab 12/02/2004 14108
Openlab Module - 3D Rendering - using the 'Scale with Z' option Openlab 12/02/2004 5823
Volocity - Alignment tool Volocity 12/02/2004 6662
Leica DMSTC Motorised XY Stage and CTRMIC microscopes in Openlab Openlab 12/02/2004 8710
ORBIT 1 XY Stage Openlab 12/02/2004 6672
An example automation illustrating the use of multithreading Openlab 12/02/2004 5754
An example automation illustrating the use of parallel processing and the "Key wait" task Openlab 12/02/2004 5783
How to create an automation to measure objects and put the results into a graph. Openlab 12/02/2004 5568
Openlab Module - Colocalization Openlab 12/02/2004 6081
Openlab Module - Image Splitter Openlab 12/02/2004 5765
Spatial calibration in Openlab 3.1 and higher Openlab 12/02/2004 5841
How to install Openlab Darkroom Openlab 12/02/2004 6301
QImaging Camera Firmware Upgrade Openlab 12/02/2004 6544
Openlab Ratio Module: Intensity Modulated image Openlab 12/02/2004 5917
Openlab Module - FRET Openlab 12/02/2004 5948
Elongation of Spherical Structures in Z Other 12/02/2004 6737
Volocity LE 2.5 and higher Volocity 12/02/2004 5890
Openlab Automator Serial Communications Openlab 12/02/2004 6003