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Latest Tips

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Movie making in Volocity: a video tutorial

Movie making in Volocity: a video tutorial

Hi folks,

Well, I have something a bit different for you this month. As my tips that include aspects of movie making always go down well, I have decided to go back to basics and show you how to make your own movie in Volocity from...Read More

How do I make a movie that shows tracking of objects over time?

How do I make a movie that shows tracking of objects over time?

Everyone loves a good movie and a question that I am frequently asked is how I make movies in Volocity that show tracking of objects as they move in time. These movies help to give a more detailed insight into biological processes and they are great for incorporating...Read More

Create a movie which plays through your Z planes at a chosen XY angle.

Create a movie which plays through your Z planes at a chosen XY angle.

A couple of weeks ago, one of my colleagues asked me how he could create a movie in Volocity which would allow him to play through his Z-stack in the 3D planes view using a chosen XY angle, as he wanted...Read More

Minimizing axial elongation of structures

Minimizing axial elongation of structures

I have a short tip for you this month, inspired by a conversation with one of my grad students.

In our lab, we use fluorescent beads to test the performance and calibration of our system and to measuring point spread functions (PSFs) to model the behaviour of light. The...Read More

Technical Notes

Title Product Modification Date Views
Columbus Software Installation Guide Columbus 02/10/2014 3238
Columbus 2.5 and Acapella Script Compatibility Columbus 19/09/2014 2073
Volocity Supported Hardware Volocity 19/07/2013 21275
What's New in Volocity 6.3 Volocity 17/07/2013 2753
Exporting TIFF Data from Volocity Volocity 01/07/2013 2603
Olympus IX83 Driver Installation Volocity 08/05/2013 3146
Workflows for In-depth High Content Analysis Columbus,Volocity 23/01/2013 2917
Data Transfer from Operetta to Volocity Volocity 21/01/2013 2761
Data Transfer from Columbus to Volocity Columbus,Volocity 21/01/2013 2671
Data Transfer from Operetta to Columbus Columbus 21/01/2013 2887
Data Transfer from Opera to Columbus Columbus 21/01/2013 2727
Data Transfer from Opera to Volocity Volocity 21/01/2013 2621
System Requirements for Volocity Product Range Volocity 22/11/2012 19861
Using the Nikon TE2000 PFS with Volocity Acquisition Volocity 22/11/2012 5299
Using the Leica DMI6000 AFC with Volocity Acquisition Volocity 22/11/2012 3772
Using the Nikon TiE PFS with Volocity Acquisition Volocity 21/11/2012 5088
What's New in Volocity 6.2? Volocity 21/11/2012 2923
Keyboard Shortcuts in Volocity Volocity 11/06/2012 4019
What's New in Volocity 6.1? Volocity 23/04/2012 3121
Skeletal Length Measurement in Volocity 6.0 Volocity 13/09/2011 3438
What's New in Volocity 6.0? Volocity 13/09/2011 3463
Openlab Supported Hardware Openlab 15/03/2011 16796
Configuring Volocity Acquisition Systems for Stitching Volocity 15/03/2011 4487
Creating and Editing Complex ROIs in Volocity 5.5 Volocity 10/01/2011 3849
Using the Zeiss AxioObserver Definite Focus with Volocity Acquisition Volocity 13/12/2010 5489
What's New in Volocity 5.5? Volocity 09/12/2010 4040
Using Lightpaths with the UltraVIEW Volocity 18/11/2010 3714
Calibrating PK Unit on Upgraded UltraVIEW LCI System Volocity 11/08/2010 3924
Volocity Demo Getting Started Guide Volocity 04/08/2010 7806
Volocity Getting started Volocity 04/08/2010 4351
Volocity and Windows 7 Volocity 28/05/2010 4739
Using the Olympus IX81 ZDC with Volocity Acquisition Volocity 27/05/2010 5709
Limitations of Hamamatsu ImagEM "Real-time Image Processing" Features Other 07/12/2009 4861
UltraVIEW VoX - Optimizing Acquisition Frame Rate Volocity 09/07/2009 4820
Volocity for Windows x64 Volocity 15/04/2009 6792
Volocity Surface Area Measurements Volocity 14/01/2009 4697
Volocity and Windows Vista Volocity 17/11/2008 5960
ILS System Requirements ILS 13/08/2008 7076
Additional TIFF Information in Phylum and Volocity Phylum and Volocity 11/06/2008 6910
System Specifications for Imaging Computing Server Volocity 02/04/2008 6175
Openlab 5.5 Openlab 27/03/2008 5154
Volocity for Windows: Optimizing performance Volocity 28/02/2008 7941
Spectral Separation of Monochrome Images Using Volocity 4 Volocity 13/08/2007 5260
Volocity and Neurolucida Volocity 30/03/2007 5452
Openlab 5: Color Tables and Automator Items Openlab 21/12/2006 5790
Finding Dirt and Debris in your Microscope Other 24/11/2006 7410
Diagnosing Microscope Drift Problems Openlab 22/11/2006 9846
Improvision Micro-Shutter performance Phylum Live 02/05/2006 4460
Openlab 5: New Location for Color Tables, Automator Menu Items and Automator Subroutines Openlab 28/04/2006 5657
Openlab and Mac OS X Openlab 16/01/2006 13660
Improvision Micro-Shutter performance Other 04/01/2006 9422
A study of fluorescence standards confirms that OptiGrid confocal images are suitable for quantitative microscopy Phylum Live 22/08/2005 4670
How do I transfer files between a PC and a Mac? Openlab 19/08/2005 14265
How does Openlab work with different image formats? Openlab 19/08/2005 6174
Which image files are compatible with Openlab? Openlab 19/08/2005 7497
How can I open TIFF Images with Openlab? Openlab 19/08/2005 6194
Why does there appear to be degradation to the resolution of the graphics in an annotation layer compared to that of a measurement overlay? Openlab 19/08/2005 6339
How can I create my own columns in the Advanced Measurements table? Openlab 19/08/2005 5914
What is a typical practical value for the accuracy and repeatability of the PIFOC piezo focus drive? Openlab 19/08/2005 6296
How do I export images from Openlab as individual TIFFs for use in other graphics applications? Openlab 19/08/2005 7472
How do I optimise memory in Mac OS 9 for use with Openlab? Openlab 19/08/2005 6069
How do I save the color LUT when exporting images into Adobe Photoshop? Openlab 19/08/2005 6987
Why is the device connected to the Serial Port of my computer not communicating with Openlab? Openlab 19/08/2005 6292
Why am I only able to capture images at a reduced size rather than full image size? Openlab 19/08/2005 5443
Controlling the VMM-D3 Three Channel Shutter Driver using Openlab Automator Serial Communications Openlab 19/08/2005 6722
Volocity for Windows - Dongle Installation Volocity 19/08/2005 7068
Using Openlab Layer User Data to set properties within Volocity Volocity 19/08/2005 5720
How do I run serial devices from a Mac which has only USB ports? Openlab 26/07/2005 5942
Year 2000 Statement Openlab 26/07/2005 5887
Achieving accurate color reproduction for brightfield microscopy using a color camer. Openlab 25/07/2005 6099
How do I make a scale bar to stamp on my images in Openlab? Openlab 25/07/2005 9651
Openlab Raw Format Openlab 09/11/2004 13577
Openlab Module - User Data Openlab 11/03/2004 5590
Ludl Controller Configuration Openlab 11/03/2004 7237
How do I density slice an image? Openlab 12/02/2004 8444
How do I edit the settings on my Openlab Filterwheel palette? Openlab 12/02/2004 5796
How do I set up the Dage DC330 color camera for transmitted light microscopy in Openlab? Openlab 12/02/2004 6039
Openlab User Interface Concepts Openlab 12/02/2004 5565
Can Phylum archive movie files? Phylum 12/02/2004 6115
How do I calibrate my TILL Photonics Polychrome monochromator in Openlab? Openlab 12/02/2004 7976
How do I use the CRI Micro Color filter with Openlab? Openlab 12/02/2004 6504
How do I prevent erratic timings when capturing large sequences of images in Openlab? Openlab 12/02/2004 5636
Does the Openlab Automator support array variables? Openlab 12/02/2004 5835
How do I superimpose results from the Measurements table onto an image layer? Openlab 12/02/2004 6089
How can I open images acquired with older Improvision imaging products in Openlab? Openlab 12/02/2004 5911
Power Macintosh G3 and G4 computers using Mac OS 9 with the National Instruments PCI 1200 card. Openlab 12/02/2004 6018
How do I measure the optical density of objects using Openlab? Openlab 12/02/2004 8763
How do I set up and use the the multi-user facility in Openlab? Openlab 12/02/2004 5236
How can I record a variable in an Openlab automation to be exported to a data analysis program such as Excel? Openlab 12/02/2004 5573
Where can I find the Openlab serial number? Openlab 12/02/2004 6162
Why do Openlab images look poor on the monitor? Openlab 12/02/2004 5339
Why does my automation refuse to stop during a Tell or Input task? Openlab 12/02/2004 5428
Why does my automation refuse to pause during a Tell task? Openlab 12/02/2004 5435
How can I determine the exact degree of image registration that is needed to re-align my multilabelled fluorescent image Openlab 12/02/2004 6718
Why can't I import Adobe Photoshop files into Phylum? Phylum 12/02/2004 5764
How do I move images from different Openlab documents into one Openlab document? Openlab 12/02/2004 5497
Keyboard Shortcuts for use with Openlab. Openlab 12/02/2004 6166
How do I convert the Openlab layer time stamp from days:hours:mins:sec into seconds so that I can use the data in Excel? Openlab 12/02/2004 5776
Why can't I annotate my images in white text using a transparent annotation layer in Openlab? Openlab 12/02/2004 5535
How do I track the speed and position of cells in a time lapse sequence with Openlab? Openlab 12/02/2004 6006
How do I make a QuickTime movie in Openlab? Openlab 12/02/2004 5818
How can I make an automation go faster? Openlab 12/02/2004 5439
Why can't I annotate my images using the Paint Tools palette? Openlab 12/02/2004 5735
Why does the image sequence that I saved as a QuickTime movie look pixelated? Openlab 12/02/2004 5910
How can I export graphs generated in Openlab into other applications ? Openlab 12/02/2004 5873
How do I reset the Point Counter so that I can begin a new count on each layer? Openlab 12/02/2004 5627
I am using OpenLab with Mac OS 9.2.2 and I am repeatedly getting out of memory errors when I try different commands. What should I do? Openlab 12/02/2004 5557
I am using binning to increase the sensitivity of my camera. I can view the image in preview mode but get an error message when I try to capture the image. Openlab 12/02/2004 5524
I’m selecting an ROI measurement from the Measurements Table, but Openlab is not displaying either the ROI or the image that it corresponds to in the document window. Openlab 12/02/2004 5644
I have downloaded the latest version of Openlab from www.improvision.com. Now what do I do? Openlab 12/02/2004 5700
How do I change the color of the paint tools? Openlab 12/02/2004 5519
What should I know about the 3D Restoration module? Openlab 12/02/2004 5678
How can I focus the SPOT RT camera quickly? Openlab 12/02/2004 6134
How can I play a movie in Phylum which doesn't include all of the images in the folder? Phylum 12/02/2004 5487
Why doesn't the software recognize my camera after I have installed a new version of Openlab? Openlab 12/02/2004 5833
Why can’t I apply a color look up table (LUT) to my merged images? Openlab 12/02/2004 5852
Which way should filters go into my filter wheel? Other 12/02/2004 5602
I can't see the line that has been drawn when I create an ROI or use the measurement tools in Openlab. Can I change the color? Openlab 12/02/2004 5377
When I use the RGB merge to merge a slightly opaque layer with other layers, why is the resultant image of poor quality, and why is detail in the opaque layer lost? Openlab 12/02/2004 5826
Why is only the Layers Manager palette available when I start up Openlab? Openlab 12/02/2004 5999
Can I use 8 or 12 bit TIFF files generated by other systems within Openlab? Openlab 12/02/2004 5647
Why am I getting a grainy black and white image on my color CoolSNAP camera? Openlab 12/02/2004 5894
My camera has non symmetrical pixels. Which value do I enter for the pixel size in the deconvolution dialog box? Openlab 12/02/2004 5480
How do I use the AxioCam camera with Openlab? Openlab 12/02/2004 5998
Zeiss Axioplan 2ie and Axiovert 200M behave strangely with Openlab 2 Openlab 12/02/2004 7465
Openlab 3 - The Filters and Shutters module now supports 'link to video' for shutters. Openlab 12/02/2004 5589
Increased functionality for the Automator Lite in Openlab 3 Openlab 12/02/2004 5396
What features of the Hamamatsu 4742-95ER (Orca ER) are now supported in Openlab 2.2.5r2? Openlab 12/02/2004 6720
Auto Exposure in Openlab Openlab 12/02/2004 6129
Preferences Issues When Moving Between Openlab versions 2 and 3. Openlab 12/02/2004 5489
Openlab 3 - Auto Focus now supported for motorised focus drives Openlab 12/02/2004 5937
Using Roper (Princeton Instruments) cameras with the PVCAM Video module Openlab 12/02/2004 8705
Can I make a binary mask using the Binary Operations module? Openlab 12/02/2004 6296
Why is the integration of my Hamamatsu C5810 camera not synchronised within an automation? Openlab 12/02/2004 6383
How do I save spatial calibrations for different objectives in Openlab? Openlab 12/02/2004 5489
Which cameras will speed up significantly when using the Automator "Capture Multiple" task? Openlab 12/02/2004 5687
What is the UV throughput of the fiber optic supplied with the TILL monochromators and are they solid quartz? Openlab 12/02/2004 5950
How do I use Openlab to measure the greyscale intensity values for a number of different ROIs over the course of a timelapse experiment (i.e. over several layers)? Openlab 12/02/2004 5806
I lost the power to the Openlab acquisition system and lost the image file I had captured. Can I get it back? Openlab 12/02/2004 5628
Can I use more than one Ludl controller with Openlab? Openlab 12/02/2004 5749
How do I remove the number from my cell tracking overlay in Openlab? Openlab 12/02/2004 5521
I cannot get communication with my ORBIT 1 controller in Openlab. Openlab 12/02/2004 6013
How do you make a 3D profile in Openlab? Openlab 12/02/2004 5531
How can I measure grayscale intensity values over an image using the same ROI? Openlab 12/02/2004 7359
When I draw an ROI on an image, it won't retain the shape I have drawn. Openlab 12/02/2004 5527
How do I change size of image in order to speed up capture and/or processing time? Openlab 12/02/2004 5718
Changes in Openlab 3 to the Automator Controls palette Openlab 12/02/2004 5548
"Save as Multiple" and file naming in Openlab Openlab 12/02/2004 6066
How do I measure the total intensity of a cell or ROI in Openlab? Openlab 12/02/2004 7510
What version of Mac OS can I use with Openlab? Openlab 12/02/2004 5823
Openlab 3.0.2 - Target Image Window Automator task - new option Openlab 12/02/2004 5816
Orbit Focus Drives - Preferences Openlab 12/02/2004 5881
Volocity 3D Rendering - how to export a 2D view Volocity 12/02/2004 6065
Write to User Notes Automator task - Tab function Openlab 12/02/2004 6171
Open Image Document Automator Task Openlab 12/02/2004 5732
Save Document Automator Task - format options Openlab 12/02/2004 5815
When using the "Volume Deconvolution" module in Openlab, the final deconvolved image does not resemble the image in the preview window. Openlab 12/02/2004 5459
Using Volocity, I have enhanced my images by using the Auto Contrast function but my volume does not look as expected. Volocity 12/02/2004 5576
Openlab Microscope Control - Light Path Automator task Openlab 12/02/2004 5863
Set Video Control Automator task - changing check boxes and pop up choices Openlab 12/02/2004 5545
Ludl Biopoint controllers do not fully support State Saver operations. Openlab 12/02/2004 5800
Alternate Readout Mode for Photometrics PVCAM cameras, specifically the CoolSNAP HQ. Openlab 12/02/2004 6586
Why is the progress bar on the Video Controls palette always moving? It never becomes a solid block, is this correct? Openlab 12/02/2004 5364
Zeiss AxioCam color camera - White Balance for brightfield microscopy. Openlab 12/02/2004 7894
Roper Scientific Photometrics color CoolSNAP camera - White Balance for brightfield microscopy. Openlab 12/02/2004 6569
How do I connect serial devices to Macs which do not have serial ports? Openlab 12/02/2004 13774
Openlab Module - 3D Rendering - using the 'Scale with Z' option Openlab 12/02/2004 5699
Volocity - Alignment tool Volocity 12/02/2004 6531
Leica DMSTC Motorised XY Stage and CTRMIC microscopes in Openlab Openlab 12/02/2004 8525
ORBIT 1 XY Stage Openlab 12/02/2004 6544
An example automation illustrating the use of multithreading Openlab 12/02/2004 5620
An example automation illustrating the use of parallel processing and the "Key wait" task Openlab 12/02/2004 5661
How to create an automation to measure objects and put the results into a graph. Openlab 12/02/2004 5449
Openlab Module - Colocalization Openlab 12/02/2004 5961
Openlab Module - Image Splitter Openlab 12/02/2004 5644
Spatial calibration in Openlab 3.1 and higher Openlab 12/02/2004 5720
How to install Openlab Darkroom Openlab 12/02/2004 6164
QImaging Camera Firmware Upgrade Openlab 12/02/2004 6406
Openlab Ratio Module: Intensity Modulated image Openlab 12/02/2004 5804
Openlab Module - FRET Openlab 12/02/2004 5827
Elongation of Spherical Structures in Z Other 12/02/2004 6607
Volocity LE 2.5 and higher Volocity 12/02/2004 5758
Openlab Automator Serial Communications Openlab 12/02/2004 5882