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Latest Tips

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Movie making in Volocity: a video tutorial

Movie making in Volocity: a video tutorial

Hi folks,

Well, I have something a bit different for you this month. As my tips that include aspects of movie making always go down well, I have decided to go back to basics and show you how to make your own movie in Volocity from...Read More

How do I make a movie that shows tracking of objects over time?

How do I make a movie that shows tracking of objects over time?

Everyone loves a good movie and a question that I am frequently asked is how I make movies in Volocity that show tracking of objects as they move in time. These movies help to give a more detailed insight into biological processes and they are great for incorporating...Read More

Create a movie which plays through your Z planes at a chosen XY angle.

Create a movie which plays through your Z planes at a chosen XY angle.

A couple of weeks ago, one of my colleagues asked me how he could create a movie in Volocity which would allow him to play through his Z-stack in the 3D planes view using a chosen XY angle, as he wanted...Read More

Minimizing axial elongation of structures

Minimizing axial elongation of structures

I have a short tip for you this month, inspired by a conversation with one of my grad students.

In our lab, we use fluorescent beads to test the performance and calibration of our system and to measuring point spread functions (PSFs) to model the behaviour of light. The...Read More

Technical Notes

Title Product Modification Date Views
Columbus Software Installation Guide Columbus 02/10/2014 3037
Columbus 2.5 and Acapella Script Compatibility Columbus 19/09/2014 1870
Volocity Supported Hardware Volocity 19/07/2013 21043
What's New in Volocity 6.3 Volocity 17/07/2013 2555
Exporting TIFF Data from Volocity Volocity 01/07/2013 2419
Olympus IX83 Driver Installation Volocity 08/05/2013 2954
Workflows for In-depth High Content Analysis Columbus,Volocity 23/01/2013 2714
Data Transfer from Operetta to Volocity Volocity 21/01/2013 2590
Data Transfer from Columbus to Volocity Columbus,Volocity 21/01/2013 2504
Data Transfer from Operetta to Columbus Columbus 21/01/2013 2707
Data Transfer from Opera to Columbus Columbus 21/01/2013 2542
Data Transfer from Opera to Volocity Volocity 21/01/2013 2447
System Requirements for Volocity Product Range Volocity 22/11/2012 19585
Using the Nikon TE2000 PFS with Volocity Acquisition Volocity 22/11/2012 5128
Using the Leica DMI6000 AFC with Volocity Acquisition Volocity 22/11/2012 3592
Using the Nikon TiE PFS with Volocity Acquisition Volocity 21/11/2012 4919
What's New in Volocity 6.2? Volocity 21/11/2012 2649
Keyboard Shortcuts in Volocity Volocity 11/06/2012 3808
What's New in Volocity 6.1? Volocity 23/04/2012 2948
Skeletal Length Measurement in Volocity 6.0 Volocity 13/09/2011 3244
What's New in Volocity 6.0? Volocity 13/09/2011 3282
Openlab Supported Hardware Openlab 15/03/2011 16613
Configuring Volocity Acquisition Systems for Stitching Volocity 15/03/2011 4279
Creating and Editing Complex ROIs in Volocity 5.5 Volocity 10/01/2011 3681
Using the Zeiss AxioObserver Definite Focus with Volocity Acquisition Volocity 13/12/2010 5245
What's New in Volocity 5.5? Volocity 09/12/2010 3858
Using Lightpaths with the UltraVIEW Volocity 18/11/2010 3557
Calibrating PK Unit on Upgraded UltraVIEW LCI System Volocity 11/08/2010 3754
Volocity Demo Getting Started Guide Volocity 04/08/2010 7581
Volocity Getting started Volocity 04/08/2010 4174
Volocity and Windows 7 Volocity 28/05/2010 4545
Using the Olympus IX81 ZDC with Volocity Acquisition Volocity 27/05/2010 5508
Limitations of Hamamatsu ImagEM "Real-time Image Processing" Features Other 07/12/2009 4650
UltraVIEW VoX - Optimizing Acquisition Frame Rate Volocity 09/07/2009 4622
Volocity for Windows x64 Volocity 15/04/2009 6618
Volocity Surface Area Measurements Volocity 14/01/2009 4473
Volocity and Windows Vista Volocity 17/11/2008 5774
ILS System Requirements ILS 13/08/2008 6866
Additional TIFF Information in Phylum and Volocity Phylum and Volocity 11/06/2008 6734
System Specifications for Imaging Computing Server Volocity 02/04/2008 5978
Openlab 5.5 Openlab 27/03/2008 4991
Volocity for Windows: Optimizing performance Volocity 28/02/2008 7747
Spectral Separation of Monochrome Images Using Volocity 4 Volocity 13/08/2007 5078
Volocity and Neurolucida Volocity 30/03/2007 5282
Openlab 5: Color Tables and Automator Items Openlab 21/12/2006 5596
Finding Dirt and Debris in your Microscope Other 24/11/2006 7175
Diagnosing Microscope Drift Problems Openlab 22/11/2006 9416
Improvision Micro-Shutter performance Phylum Live 02/05/2006 4273
Openlab 5: New Location for Color Tables, Automator Menu Items and Automator Subroutines Openlab 28/04/2006 5484
Openlab and Mac OS X Openlab 16/01/2006 13438
Improvision Micro-Shutter performance Other 04/01/2006 9262
A study of fluorescence standards confirms that OptiGrid confocal images are suitable for quantitative microscopy Phylum Live 22/08/2005 4489
How do I transfer files between a PC and a Mac? Openlab 19/08/2005 14072
How does Openlab work with different image formats? Openlab 19/08/2005 5999
Which image files are compatible with Openlab? Openlab 19/08/2005 7302
How can I open TIFF Images with Openlab? Openlab 19/08/2005 5996
Why does there appear to be degradation to the resolution of the graphics in an annotation layer compared to that of a measurement overlay? Openlab 19/08/2005 6169
How can I create my own columns in the Advanced Measurements table? Openlab 19/08/2005 5741
What is a typical practical value for the accuracy and repeatability of the PIFOC piezo focus drive? Openlab 19/08/2005 6123
How do I export images from Openlab as individual TIFFs for use in other graphics applications? Openlab 19/08/2005 7240
How do I optimise memory in Mac OS 9 for use with Openlab? Openlab 19/08/2005 5857
How do I save the color LUT when exporting images into Adobe Photoshop? Openlab 19/08/2005 6794
Why is the device connected to the Serial Port of my computer not communicating with Openlab? Openlab 19/08/2005 6106
Why am I only able to capture images at a reduced size rather than full image size? Openlab 19/08/2005 5273
Controlling the VMM-D3 Three Channel Shutter Driver using Openlab Automator Serial Communications Openlab 19/08/2005 6526
Volocity for Windows - Dongle Installation Volocity 19/08/2005 6828
Using Openlab Layer User Data to set properties within Volocity Volocity 19/08/2005 5534
How do I run serial devices from a Mac which has only USB ports? Openlab 26/07/2005 5764
Year 2000 Statement Openlab 26/07/2005 5697
Achieving accurate color reproduction for brightfield microscopy using a color camer. Openlab 25/07/2005 5913
How do I make a scale bar to stamp on my images in Openlab? Openlab 25/07/2005 9397
Openlab Raw Format Openlab 09/11/2004 13159
Openlab Module - User Data Openlab 11/03/2004 5418
Ludl Controller Configuration Openlab 11/03/2004 7056
How do I density slice an image? Openlab 12/02/2004 8175
How do I edit the settings on my Openlab Filterwheel palette? Openlab 12/02/2004 5598
How do I set up the Dage DC330 color camera for transmitted light microscopy in Openlab? Openlab 12/02/2004 5858
Openlab User Interface Concepts Openlab 12/02/2004 5385
Can Phylum archive movie files? Phylum 12/02/2004 5936
How do I calibrate my TILL Photonics Polychrome monochromator in Openlab? Openlab 12/02/2004 7718
How do I use the CRI Micro Color filter with Openlab? Openlab 12/02/2004 6316
How do I prevent erratic timings when capturing large sequences of images in Openlab? Openlab 12/02/2004 5454
Does the Openlab Automator support array variables? Openlab 12/02/2004 5663
How do I superimpose results from the Measurements table onto an image layer? Openlab 12/02/2004 5910
How can I open images acquired with older Improvision imaging products in Openlab? Openlab 12/02/2004 5724
Power Macintosh G3 and G4 computers using Mac OS 9 with the National Instruments PCI 1200 card. Openlab 12/02/2004 5812
How do I measure the optical density of objects using Openlab? Openlab 12/02/2004 8459
How do I set up and use the the multi-user facility in Openlab? Openlab 12/02/2004 5033
How can I record a variable in an Openlab automation to be exported to a data analysis program such as Excel? Openlab 12/02/2004 5376
Where can I find the Openlab serial number? Openlab 12/02/2004 5944
Why do Openlab images look poor on the monitor? Openlab 12/02/2004 5165
Why does my automation refuse to stop during a Tell or Input task? Openlab 12/02/2004 5255
Why does my automation refuse to pause during a Tell task? Openlab 12/02/2004 5271
How can I determine the exact degree of image registration that is needed to re-align my multilabelled fluorescent image Openlab 12/02/2004 6530
Why can't I import Adobe Photoshop files into Phylum? Phylum 12/02/2004 5584
How do I move images from different Openlab documents into one Openlab document? Openlab 12/02/2004 5330
Keyboard Shortcuts for use with Openlab. Openlab 12/02/2004 5946
How do I convert the Openlab layer time stamp from days:hours:mins:sec into seconds so that I can use the data in Excel? Openlab 12/02/2004 5578
Why can't I annotate my images in white text using a transparent annotation layer in Openlab? Openlab 12/02/2004 5365
How do I track the speed and position of cells in a time lapse sequence with Openlab? Openlab 12/02/2004 5829
How do I make a QuickTime movie in Openlab? Openlab 12/02/2004 5619
How can I make an automation go faster? Openlab 12/02/2004 5258
Why can't I annotate my images using the Paint Tools palette? Openlab 12/02/2004 5536
Why does the image sequence that I saved as a QuickTime movie look pixelated? Openlab 12/02/2004 5724
How can I export graphs generated in Openlab into other applications ? Openlab 12/02/2004 5664
How do I reset the Point Counter so that I can begin a new count on each layer? Openlab 12/02/2004 5458
I am using OpenLab with Mac OS 9.2.2 and I am repeatedly getting out of memory errors when I try different commands. What should I do? Openlab 12/02/2004 5361
I am using binning to increase the sensitivity of my camera. I can view the image in preview mode but get an error message when I try to capture the image. Openlab 12/02/2004 5341
I’m selecting an ROI measurement from the Measurements Table, but Openlab is not displaying either the ROI or the image that it corresponds to in the document window. Openlab 12/02/2004 5458
I have downloaded the latest version of Openlab from www.improvision.com. Now what do I do? Openlab 12/02/2004 5508
How do I change the color of the paint tools? Openlab 12/02/2004 5329
What should I know about the 3D Restoration module? Openlab 12/02/2004 5503
How can I focus the SPOT RT camera quickly? Openlab 12/02/2004 5887
How can I play a movie in Phylum which doesn't include all of the images in the folder? Phylum 12/02/2004 5320
Why doesn't the software recognize my camera after I have installed a new version of Openlab? Openlab 12/02/2004 5627
Why can’t I apply a color look up table (LUT) to my merged images? Openlab 12/02/2004 5671
Which way should filters go into my filter wheel? Other 12/02/2004 5432
I can't see the line that has been drawn when I create an ROI or use the measurement tools in Openlab. Can I change the color? Openlab 12/02/2004 5238
When I use the RGB merge to merge a slightly opaque layer with other layers, why is the resultant image of poor quality, and why is detail in the opaque layer lost? Openlab 12/02/2004 5651
Why is only the Layers Manager palette available when I start up Openlab? Openlab 12/02/2004 5805
Can I use 8 or 12 bit TIFF files generated by other systems within Openlab? Openlab 12/02/2004 5477
Why am I getting a grainy black and white image on my color CoolSNAP camera? Openlab 12/02/2004 5712
My camera has non symmetrical pixels. Which value do I enter for the pixel size in the deconvolution dialog box? Openlab 12/02/2004 5306
How do I use the AxioCam camera with Openlab? Openlab 12/02/2004 5803
Zeiss Axioplan 2ie and Axiovert 200M behave strangely with Openlab 2 Openlab 12/02/2004 7252
Openlab 3 - The Filters and Shutters module now supports 'link to video' for shutters. Openlab 12/02/2004 5426
Increased functionality for the Automator Lite in Openlab 3 Openlab 12/02/2004 5231
What features of the Hamamatsu 4742-95ER (Orca ER) are now supported in Openlab 2.2.5r2? Openlab 12/02/2004 6534
Auto Exposure in Openlab Openlab 12/02/2004 5937
Preferences Issues When Moving Between Openlab versions 2 and 3. Openlab 12/02/2004 5311
Openlab 3 - Auto Focus now supported for motorised focus drives Openlab 12/02/2004 5745
Using Roper (Princeton Instruments) cameras with the PVCAM Video module Openlab 12/02/2004 8340
Can I make a binary mask using the Binary Operations module? Openlab 12/02/2004 6097
Why is the integration of my Hamamatsu C5810 camera not synchronised within an automation? Openlab 12/02/2004 6207
How do I save spatial calibrations for different objectives in Openlab? Openlab 12/02/2004 5308
Which cameras will speed up significantly when using the Automator "Capture Multiple" task? Openlab 12/02/2004 5503
What is the UV throughput of the fiber optic supplied with the TILL monochromators and are they solid quartz? Openlab 12/02/2004 5772
How do I use Openlab to measure the greyscale intensity values for a number of different ROIs over the course of a timelapse experiment (i.e. over several layers)? Openlab 12/02/2004 5617
I lost the power to the Openlab acquisition system and lost the image file I had captured. Can I get it back? Openlab 12/02/2004 5457
Can I use more than one Ludl controller with Openlab? Openlab 12/02/2004 5564
How do I remove the number from my cell tracking overlay in Openlab? Openlab 12/02/2004 5352
I cannot get communication with my ORBIT 1 controller in Openlab. Openlab 12/02/2004 5842
How do you make a 3D profile in Openlab? Openlab 12/02/2004 5333
How can I measure grayscale intensity values over an image using the same ROI? Openlab 12/02/2004 7141
When I draw an ROI on an image, it won't retain the shape I have drawn. Openlab 12/02/2004 5344
How do I change size of image in order to speed up capture and/or processing time? Openlab 12/02/2004 5545
Changes in Openlab 3 to the Automator Controls palette Openlab 12/02/2004 5370
"Save as Multiple" and file naming in Openlab Openlab 12/02/2004 5889
How do I measure the total intensity of a cell or ROI in Openlab? Openlab 12/02/2004 7099
What version of Mac OS can I use with Openlab? Openlab 12/02/2004 5636
Openlab 3.0.2 - Target Image Window Automator task - new option Openlab 12/02/2004 5621
Orbit Focus Drives - Preferences Openlab 12/02/2004 5693
Volocity 3D Rendering - how to export a 2D view Volocity 12/02/2004 5879
Write to User Notes Automator task - Tab function Openlab 12/02/2004 5990
Open Image Document Automator Task Openlab 12/02/2004 5532
Save Document Automator Task - format options Openlab 12/02/2004 5637
When using the "Volume Deconvolution" module in Openlab, the final deconvolved image does not resemble the image in the preview window. Openlab 12/02/2004 5280
Using Volocity, I have enhanced my images by using the Auto Contrast function but my volume does not look as expected. Volocity 12/02/2004 5406
Openlab Microscope Control - Light Path Automator task Openlab 12/02/2004 5673
Set Video Control Automator task - changing check boxes and pop up choices Openlab 12/02/2004 5365
Ludl Biopoint controllers do not fully support State Saver operations. Openlab 12/02/2004 5610
Alternate Readout Mode for Photometrics PVCAM cameras, specifically the CoolSNAP HQ. Openlab 12/02/2004 6373
Why is the progress bar on the Video Controls palette always moving? It never becomes a solid block, is this correct? Openlab 12/02/2004 5189
Zeiss AxioCam color camera - White Balance for brightfield microscopy. Openlab 12/02/2004 7629
Roper Scientific Photometrics color CoolSNAP camera - White Balance for brightfield microscopy. Openlab 12/02/2004 6379
How do I connect serial devices to Macs which do not have serial ports? Openlab 12/02/2004 13406
Openlab Module - 3D Rendering - using the 'Scale with Z' option Openlab 12/02/2004 5503
Volocity - Alignment tool Volocity 12/02/2004 6354
Leica DMSTC Motorised XY Stage and CTRMIC microscopes in Openlab Openlab 12/02/2004 8225
ORBIT 1 XY Stage Openlab 12/02/2004 6348
An example automation illustrating the use of multithreading Openlab 12/02/2004 5420
An example automation illustrating the use of parallel processing and the "Key wait" task Openlab 12/02/2004 5481
How to create an automation to measure objects and put the results into a graph. Openlab 12/02/2004 5268
Openlab Module - Colocalization Openlab 12/02/2004 5774
Openlab Module - Image Splitter Openlab 12/02/2004 5451
Spatial calibration in Openlab 3.1 and higher Openlab 12/02/2004 5537
How to install Openlab Darkroom Openlab 12/02/2004 5951
QImaging Camera Firmware Upgrade Openlab 12/02/2004 6193
Openlab Ratio Module: Intensity Modulated image Openlab 12/02/2004 5628
Openlab Module - FRET Openlab 12/02/2004 5639
Elongation of Spherical Structures in Z Other 12/02/2004 6404
Volocity LE 2.5 and higher Volocity 12/02/2004 5573
Openlab Automator Serial Communications Openlab 12/02/2004 5697