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Latest Tips

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Movie making in Volocity: a video tutorial

Movie making in Volocity: a video tutorial

Hi folks,

Well, I have something a bit different for you this month. As my tips that include aspects of movie making always go down well, I have decided to go back to basics and show you how to make your own movie in Volocity from...Read More

How do I make a movie that shows tracking of objects over time?

How do I make a movie that shows tracking of objects over time?

Everyone loves a good movie and a question that I am frequently asked is how I make movies in Volocity that show tracking of objects as they move in time. These movies help to give a more detailed insight into biological processes and they are great for incorporating...Read More

Create a movie which plays through your Z planes at a chosen XY angle.

Create a movie which plays through your Z planes at a chosen XY angle.

A couple of weeks ago, one of my colleagues asked me how he could create a movie in Volocity which would allow him to play through his Z-stack in the 3D planes view using a chosen XY angle, as he wanted...Read More

Minimizing axial elongation of structures

Minimizing axial elongation of structures

I have a short tip for you this month, inspired by a conversation with one of my grad students.

In our lab, we use fluorescent beads to test the performance and calibration of our system and to measuring point spread functions (PSFs) to model the behaviour of light. The...Read More

Technical Notes

Title Product Modification Date Views
Columbus Software Installation Guide Columbus 02/10/2014 2456
Columbus 2.5 and Acapella Script Compatibility Columbus 19/09/2014 1278
Volocity Supported Hardware Volocity 19/07/2013 20334
What's New in Volocity 6.3 Volocity 17/07/2013 1978
Exporting TIFF Data from Volocity Volocity 01/07/2013 1816
Olympus IX83 Driver Installation Volocity 08/05/2013 2343
Workflows for In-depth High Content Analysis Columbus,Volocity 23/01/2013 2143
Data Transfer from Operetta to Volocity Volocity 21/01/2013 2035
Data Transfer from Columbus to Volocity Columbus,Volocity 21/01/2013 1995
Data Transfer from Operetta to Columbus Columbus 21/01/2013 2145
Data Transfer from Opera to Columbus Columbus 21/01/2013 1999
Data Transfer from Opera to Volocity Volocity 21/01/2013 1931
System Requirements for Volocity Product Range Volocity 22/11/2012 18694
Using the Nikon TE2000 PFS with Volocity Acquisition Volocity 22/11/2012 4621
Using the Leica DMI6000 AFC with Volocity Acquisition Volocity 22/11/2012 3041
Using the Nikon TiE PFS with Volocity Acquisition Volocity 21/11/2012 4351
What's New in Volocity 6.2? Volocity 21/11/2012 2105
Keyboard Shortcuts in Volocity Volocity 11/06/2012 3202
What's New in Volocity 6.1? Volocity 23/04/2012 2406
Skeletal Length Measurement in Volocity 6.0 Volocity 13/09/2011 2698
What's New in Volocity 6.0? Volocity 13/09/2011 2729
Openlab Supported Hardware Openlab 15/03/2011 16054
Configuring Volocity Acquisition Systems for Stitching Volocity 15/03/2011 3625
Creating and Editing Complex ROIs in Volocity 5.5 Volocity 10/01/2011 3151
Using the Zeiss AxioObserver Definite Focus with Volocity Acquisition Volocity 13/12/2010 4588
What's New in Volocity 5.5? Volocity 09/12/2010 3293
Using Lightpaths with the UltraVIEW Volocity 18/11/2010 3024
Calibrating PK Unit on Upgraded UltraVIEW LCI System Volocity 11/08/2010 3233
Volocity Demo Getting Started Guide Volocity 04/08/2010 6906
Volocity Getting started Volocity 04/08/2010 3617
Volocity and Windows 7 Volocity 28/05/2010 3982
Using the Olympus IX81 ZDC with Volocity Acquisition Volocity 27/05/2010 4950
Limitations of Hamamatsu ImagEM "Real-time Image Processing" Features Other 07/12/2009 4063
UltraVIEW VoX - Optimizing Acquisition Frame Rate Volocity 09/07/2009 4039
Volocity for Windows x64 Volocity 15/04/2009 6081
Volocity Surface Area Measurements Volocity 14/01/2009 3853
Volocity and Windows Vista Volocity 17/11/2008 5225
ILS System Requirements ILS 13/08/2008 6276
Additional TIFF Information in Phylum and Volocity Phylum and Volocity 11/06/2008 6198
System Specifications for Imaging Computing Server Volocity 02/04/2008 5390
Openlab 5.5 Openlab 27/03/2008 4453
Volocity for Windows: Optimizing performance Volocity 28/02/2008 7193
Spectral Separation of Monochrome Images Using Volocity 4 Volocity 13/08/2007 4557
Volocity and Neurolucida Volocity 30/03/2007 4728
Openlab 5: Color Tables and Automator Items Openlab 21/12/2006 5026
Finding Dirt and Debris in your Microscope Other 24/11/2006 6430
Diagnosing Microscope Drift Problems Openlab 22/11/2006 7975
Improvision Micro-Shutter performance Phylum Live 02/05/2006 3716
Openlab 5: New Location for Color Tables, Automator Menu Items and Automator Subroutines Openlab 28/04/2006 4929
Openlab and Mac OS X Openlab 16/01/2006 12757
Improvision Micro-Shutter performance Other 04/01/2006 8713
A study of fluorescence standards confirms that OptiGrid confocal images are suitable for quantitative microscopy Phylum Live 22/08/2005 3939
How do I transfer files between a PC and a Mac? Openlab 19/08/2005 13487
How does Openlab work with different image formats? Openlab 19/08/2005 5450
Which image files are compatible with Openlab? Openlab 19/08/2005 6714
How can I open TIFF Images with Openlab? Openlab 19/08/2005 5415
Why does there appear to be degradation to the resolution of the graphics in an annotation layer compared to that of a measurement overlay? Openlab 19/08/2005 5597
How can I create my own columns in the Advanced Measurements table? Openlab 19/08/2005 5223
What is a typical practical value for the accuracy and repeatability of the PIFOC piezo focus drive? Openlab 19/08/2005 5583
How do I export images from Openlab as individual TIFFs for use in other graphics applications? Openlab 19/08/2005 6500
How do I optimise memory in Mac OS 9 for use with Openlab? Openlab 19/08/2005 5253
How do I save the color LUT when exporting images into Adobe Photoshop? Openlab 19/08/2005 6207
Why is the device connected to the Serial Port of my computer not communicating with Openlab? Openlab 19/08/2005 5530
Why am I only able to capture images at a reduced size rather than full image size? Openlab 19/08/2005 4738
Controlling the VMM-D3 Three Channel Shutter Driver using Openlab Automator Serial Communications Openlab 19/08/2005 5921
Volocity for Windows - Dongle Installation Volocity 19/08/2005 6075
Using Openlab Layer User Data to set properties within Volocity Volocity 19/08/2005 4988
How do I run serial devices from a Mac which has only USB ports? Openlab 26/07/2005 5213
Year 2000 Statement Openlab 26/07/2005 5119
Achieving accurate color reproduction for brightfield microscopy using a color camer. Openlab 25/07/2005 5344
How do I make a scale bar to stamp on my images in Openlab? Openlab 25/07/2005 8661
Openlab Raw Format Openlab 09/11/2004 12015
Openlab Module - User Data Openlab 11/03/2004 4887
Ludl Controller Configuration Openlab 11/03/2004 6498
How do I density slice an image? Openlab 12/02/2004 7420
How do I edit the settings on my Openlab Filterwheel palette? Openlab 12/02/2004 5074
How do I set up the Dage DC330 color camera for transmitted light microscopy in Openlab? Openlab 12/02/2004 5294
Openlab User Interface Concepts Openlab 12/02/2004 4902
Can Phylum archive movie files? Phylum 12/02/2004 5345
How do I calibrate my TILL Photonics Polychrome monochromator in Openlab? Openlab 12/02/2004 6985
How do I use the CRI Micro Color filter with Openlab? Openlab 12/02/2004 5702
How do I prevent erratic timings when capturing large sequences of images in Openlab? Openlab 12/02/2004 4921
Does the Openlab Automator support array variables? Openlab 12/02/2004 5152
How do I superimpose results from the Measurements table onto an image layer? Openlab 12/02/2004 5368
How can I open images acquired with older Improvision imaging products in Openlab? Openlab 12/02/2004 5169
Power Macintosh G3 and G4 computers using Mac OS 9 with the National Instruments PCI 1200 card. Openlab 12/02/2004 5256
How do I measure the optical density of objects using Openlab? Openlab 12/02/2004 7609
How do I set up and use the the multi-user facility in Openlab? Openlab 12/02/2004 4507
How can I record a variable in an Openlab automation to be exported to a data analysis program such as Excel? Openlab 12/02/2004 4792
Where can I find the Openlab serial number? Openlab 12/02/2004 5343
Why do Openlab images look poor on the monitor? Openlab 12/02/2004 4625
Why does my automation refuse to stop during a Tell or Input task? Openlab 12/02/2004 4732
Why does my automation refuse to pause during a Tell task? Openlab 12/02/2004 4720
How can I determine the exact degree of image registration that is needed to re-align my multilabelled fluorescent image Openlab 12/02/2004 5932
Why can't I import Adobe Photoshop files into Phylum? Phylum 12/02/2004 5039
How do I move images from different Openlab documents into one Openlab document? Openlab 12/02/2004 4787
Keyboard Shortcuts for use with Openlab. Openlab 12/02/2004 5355
How do I convert the Openlab layer time stamp from days:hours:mins:sec into seconds so that I can use the data in Excel? Openlab 12/02/2004 5005
Why can't I annotate my images in white text using a transparent annotation layer in Openlab? Openlab 12/02/2004 4855
How do I track the speed and position of cells in a time lapse sequence with Openlab? Openlab 12/02/2004 5293
How do I make a QuickTime movie in Openlab? Openlab 12/02/2004 5059
How can I make an automation go faster? Openlab 12/02/2004 4710
Why can't I annotate my images using the Paint Tools palette? Openlab 12/02/2004 4933
Why does the image sequence that I saved as a QuickTime movie look pixelated? Openlab 12/02/2004 5175
How can I export graphs generated in Openlab into other applications ? Openlab 12/02/2004 5042
How do I reset the Point Counter so that I can begin a new count on each layer? Openlab 12/02/2004 4916
I am using OpenLab with Mac OS 9.2.2 and I am repeatedly getting out of memory errors when I try different commands. What should I do? Openlab 12/02/2004 4740
I am using binning to increase the sensitivity of my camera. I can view the image in preview mode but get an error message when I try to capture the image. Openlab 12/02/2004 4792
I’m selecting an ROI measurement from the Measurements Table, but Openlab is not displaying either the ROI or the image that it corresponds to in the document window. Openlab 12/02/2004 4926
I have downloaded the latest version of Openlab from www.improvision.com. Now what do I do? Openlab 12/02/2004 4868
How do I change the color of the paint tools? Openlab 12/02/2004 4766
What should I know about the 3D Restoration module? Openlab 12/02/2004 4973
How can I focus the SPOT RT camera quickly? Openlab 12/02/2004 5324
How can I play a movie in Phylum which doesn't include all of the images in the folder? Phylum 12/02/2004 4810
Why doesn't the software recognize my camera after I have installed a new version of Openlab? Openlab 12/02/2004 5052
Why can’t I apply a color look up table (LUT) to my merged images? Openlab 12/02/2004 5097
Which way should filters go into my filter wheel? Other 12/02/2004 4878
I can't see the line that has been drawn when I create an ROI or use the measurement tools in Openlab. Can I change the color? Openlab 12/02/2004 4756
When I use the RGB merge to merge a slightly opaque layer with other layers, why is the resultant image of poor quality, and why is detail in the opaque layer lost? Openlab 12/02/2004 5088
Why is only the Layers Manager palette available when I start up Openlab? Openlab 12/02/2004 5278
Can I use 8 or 12 bit TIFF files generated by other systems within Openlab? Openlab 12/02/2004 4950
Why am I getting a grainy black and white image on my color CoolSNAP camera? Openlab 12/02/2004 5172
My camera has non symmetrical pixels. Which value do I enter for the pixel size in the deconvolution dialog box? Openlab 12/02/2004 4774
How do I use the AxioCam camera with Openlab? Openlab 12/02/2004 5239
Zeiss Axioplan 2ie and Axiovert 200M behave strangely with Openlab 2 Openlab 12/02/2004 6685
Openlab 3 - The Filters and Shutters module now supports 'link to video' for shutters. Openlab 12/02/2004 4894
Increased functionality for the Automator Lite in Openlab 3 Openlab 12/02/2004 4692
What features of the Hamamatsu 4742-95ER (Orca ER) are now supported in Openlab 2.2.5r2? Openlab 12/02/2004 5926
Auto Exposure in Openlab Openlab 12/02/2004 5354
Preferences Issues When Moving Between Openlab versions 2 and 3. Openlab 12/02/2004 4756
Openlab 3 - Auto Focus now supported for motorised focus drives Openlab 12/02/2004 5141
Using Roper (Princeton Instruments) cameras with the PVCAM Video module Openlab 12/02/2004 7580
Can I make a binary mask using the Binary Operations module? Openlab 12/02/2004 5551
Why is the integration of my Hamamatsu C5810 camera not synchronised within an automation? Openlab 12/02/2004 5625
How do I save spatial calibrations for different objectives in Openlab? Openlab 12/02/2004 4760
Which cameras will speed up significantly when using the Automator "Capture Multiple" task? Openlab 12/02/2004 4950
What is the UV throughput of the fiber optic supplied with the TILL monochromators and are they solid quartz? Openlab 12/02/2004 5214
How do I use Openlab to measure the greyscale intensity values for a number of different ROIs over the course of a timelapse experiment (i.e. over several layers)? Openlab 12/02/2004 5048
I lost the power to the Openlab acquisition system and lost the image file I had captured. Can I get it back? Openlab 12/02/2004 4913
Can I use more than one Ludl controller with Openlab? Openlab 12/02/2004 4974
How do I remove the number from my cell tracking overlay in Openlab? Openlab 12/02/2004 4790
I cannot get communication with my ORBIT 1 controller in Openlab. Openlab 12/02/2004 5337
How do you make a 3D profile in Openlab? Openlab 12/02/2004 4780
How can I measure grayscale intensity values over an image using the same ROI? Openlab 12/02/2004 6379
When I draw an ROI on an image, it won't retain the shape I have drawn. Openlab 12/02/2004 4793
How do I change size of image in order to speed up capture and/or processing time? Openlab 12/02/2004 4991
Changes in Openlab 3 to the Automator Controls palette Openlab 12/02/2004 4815
"Save as Multiple" and file naming in Openlab Openlab 12/02/2004 5328
How do I measure the total intensity of a cell or ROI in Openlab? Openlab 12/02/2004 6173
What version of Mac OS can I use with Openlab? Openlab 12/02/2004 5080
Openlab 3.0.2 - Target Image Window Automator task - new option Openlab 12/02/2004 5069
Orbit Focus Drives - Preferences Openlab 12/02/2004 5109
Volocity 3D Rendering - how to export a 2D view Volocity 12/02/2004 5247
Write to User Notes Automator task - Tab function Openlab 12/02/2004 5394
Open Image Document Automator Task Openlab 12/02/2004 4958
Save Document Automator Task - format options Openlab 12/02/2004 5049
When using the "Volume Deconvolution" module in Openlab, the final deconvolved image does not resemble the image in the preview window. Openlab 12/02/2004 4706
Using Volocity, I have enhanced my images by using the Auto Contrast function but my volume does not look as expected. Volocity 12/02/2004 4847
Openlab Microscope Control - Light Path Automator task Openlab 12/02/2004 5090
Set Video Control Automator task - changing check boxes and pop up choices Openlab 12/02/2004 4801
Ludl Biopoint controllers do not fully support State Saver operations. Openlab 12/02/2004 5074
Alternate Readout Mode for Photometrics PVCAM cameras, specifically the CoolSNAP HQ. Openlab 12/02/2004 5727
Why is the progress bar on the Video Controls palette always moving? It never becomes a solid block, is this correct? Openlab 12/02/2004 4641
Zeiss AxioCam color camera - White Balance for brightfield microscopy. Openlab 12/02/2004 6866
Roper Scientific Photometrics color CoolSNAP camera - White Balance for brightfield microscopy. Openlab 12/02/2004 5770
How do I connect serial devices to Macs which do not have serial ports? Openlab 12/02/2004 12323
Openlab Module - 3D Rendering - using the 'Scale with Z' option Openlab 12/02/2004 4946
Volocity - Alignment tool Volocity 12/02/2004 5762
Leica DMSTC Motorised XY Stage and CTRMIC microscopes in Openlab Openlab 12/02/2004 7361
ORBIT 1 XY Stage Openlab 12/02/2004 5779
An example automation illustrating the use of multithreading Openlab 12/02/2004 4835
An example automation illustrating the use of parallel processing and the "Key wait" task Openlab 12/02/2004 4920
How to create an automation to measure objects and put the results into a graph. Openlab 12/02/2004 4698
Openlab Module - Colocalization Openlab 12/02/2004 5187
Openlab Module - Image Splitter Openlab 12/02/2004 4897
Spatial calibration in Openlab 3.1 and higher Openlab 12/02/2004 5008
How to install Openlab Darkroom Openlab 12/02/2004 5302
QImaging Camera Firmware Upgrade Openlab 12/02/2004 5549
Openlab Ratio Module: Intensity Modulated image Openlab 12/02/2004 5080
Openlab Module - FRET Openlab 12/02/2004 5079
Elongation of Spherical Structures in Z Other 12/02/2004 5795
Volocity LE 2.5 and higher Volocity 12/02/2004 5038
Openlab Automator Serial Communications Openlab 12/02/2004 5141