How to set up a timelapse acquisition experiment with Volocity Acquisition.

Did you know that you can now acquire images with Volocity on both PC and Mac platforms using the optional module Volocity Acquisition?  Of course you did!  So this month here's a crash course in how you go about setting up a simple two channel timelapse experiment using Volocity Acquisition.

Let's say for instance that I want to capture two fluorescent channels – it's quite easy to setup. The first thing you would need to think about is setting up a Light Path for each channel.

From the 'Window' menu choose 'Show Video Preview'. You will find the Light Path Manager just above the device controls.

 

First configure the video settings and device settings that you want to use for the first channel.

Either double click on the Light Path or right click and select 'Edit' from the popup menu.

Before you ask the screen shots are taken from Volocity running on Windows XP.

The next step is to setup the Light Path properties, see the dialog below.

Click on the devices that you want to include in this Light Path.  As you can see I have kept this fairly simple only including 'Exposure' and the 'Turret' position.  I want to ensure that the shutters are open when this Light Path is applied so I have chosen to open the Fluorescent shutter.

After saving this dialog use the device controls to configure the Video and other device settings for the second channel.

Double click on another Light Path and save the settings.

The Light Paths are now ready to be used.

Next you need to think about how frequently you would like to acquire the images. Just above the Light Path settings you will see the timelapse settings. There are a whole range of options to pick here.

I would like to acquire a pair of images every minute so I have set it to '1 Minute per Timepoint'

Before we start the timelapse there is one more important step we need to consider, the 'Acquisition Setup'

If you go to the 'Video' menu you will find what you are looking for.

This will open the 'Acquisition Setup' dialog.

First fill in the Title, User Name and Description.

Click on the '+' button to add channels then select the appropriate light path.

The Auto-Exposure is useful but I have chosen a specific exposure that I want to use so I will leave the option disabled.

The timelapse experiment I am conducting will run for 24 hours so I will adjust the duration to cater for this.

You may have notice that just under the Duration there is a popup menu to set the shutter management.

To take full advantage of the shutter management you need to measure device latencies - I won't go into this here but if you are interested in finding out more take a look at the Volocity User's Guide which you can download from the Manuals section.

We are almost finished with the acquisition setup but you may have asked yourself “What if I want to setup a whole range of different acquisitions, can this information be saved?” Well, yes it can, in the bottom left corner of the dialog there are 'Save' and 'Restore' buttons.

 

 The 'Save button will allow you to save the settings of the acquisition setup.

Whereas the 'Restore' button will allow you to restore the settings of a saved acquisition setup.

See below, this is the one that I just saved.

Ok, that's about it for the acquisition setup of this simple two channel timelapse, click on the 'OK' button to exit the setup dialog.

When you are ready you can start the timelapse by clicking on the red button on the control panel.

I have started my timelapse, it will run for 24 hours but if I wanted to stop it early all I would have to do is click on the stop button.

I hope this is useful, see you next month.