What features of the Hamamatsu 4742-95ER (Orca ER) are now supported in Openlab 2.2.5r2?

Technical Note: 161
Reads: 7243
Creation Date: 29/01/2001
Modification Date: 12/02/2004

Openlab now has a specific configuration choice for the Orca ER camera, and supports sub-array, in the preview and normal capture, and the ability to switch between low and high light level modes.

Download Openlab 2.2.5r2 or later from the Improvision website. Install the latest version following the instructions given by the installer.

Remember to allocate RAM to the new version of the software and move over any files you are used to keeping in the Application folder, see Technical Note 139, which describes what to do when upgrading Openlab.

In Openlab, under the Special Menu, select Video Manager and from the Input menu, choose Hamamatsu Orca ER (C47642-95-ER).

You will now be able to use the full resolution of the chip (1344 x 1022 pixels). Exposure, Gain, Offset, Binnning, Digital Gain and Colorization operate as for the Orca I and Orca ER in previous versions of Openlab.

Also implemented are the sub-array features and the two light level modes.

Sub-array is a method by which an image of a size less than the maximum output of the camera is read out as efficiently and rapidly as possible. The sub-array area is chosen by reducing the document size, either from the Image menu (Image - Document Size...) or by drawing an ROI and cropping the document to that size (Edit - ROI - Crop document). Remember you can now position that sub-array within the current field of view of the chip by selecting the hand tool and holding down the alt or option key on the keyboard to drag the sub-array around. Acquisition will be fastest at the top/left of the camera frame for a given document height and width.

You can switch between the two light level modes, Low light and High light, using the pop-up menu in the Video Controls palette.

In the High light level mode, Anti-blooming is switched on so that when a well on the CCD array is saturated, charge will not overflow into the wells adjacent to it.

In the Low Light level mode, well saturation is unlikely so anti-blooming is switched off. This mode results in a higher QE (quantum efficiency) as the effective well depth is increased because it is not limited by anti-blooming. Low light level mode has a higher sensitivity and altered dynamic range compared to High light level mode. The operation of other controls is not affected.

This version does not support datastream capture (eg. using capture multiple task).