Elongation of Spherical Structures in ZTechnical Note: 382
Reads: 7313
Creation Date: 03/06/2003
Modification Date: 12/02/2004

Summary

Fluorescently labelled beads are used for a variety of purposes in optical microscopy, from measuring a point spread function to assessing the performance and calibration of the system. The beads supplied by manufacturers such as Molecular Probes are manufactured under strict controls and can be considered as spheres. This technical note discusses why 3D visualisations of these beads, reconstructed from 2D sections, appear, not as spheres, but as ovoid?


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