A study of fluorescence standards confirms that OptiGrid confocal images are suitable for quantitative microscopyTechnical Note: 425
Reads: 5409
Creation Date: 22/08/2005
Modification Date: 22/08/2005

Summary

The OptiGrid converts the illumination system of a conventional wide field microscope to a structured light illumination system. Control of Grid movement and image processing of the raw structured illumination images by a computer system allow for the generation of images with only in focus detail (Neil et al 1997). This technique effectively confers confocal capabilities to conventional wide field microscopes (Puche 2005).


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